Table 2 Comparison of the successful editing rate between Cas9-VirD2, Cas9-VirD2, and Cas9 with repair templates, with and without the RB. Both Cas9-VirD2 and VirD2-Cas9 with repair templates with the RB demonstrated an efficient rate of HDR at the target site.

From: Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice

Clones and repair templates

Fully repaired

Partial repair

Total T0 plants

Repair frequency

Cas9-VirD2 + mT-RB

8

3

81

9.87%

VirD2-Cas9 + mT-RB

3

1

31

9.67%

Cas9-VirD2 + mT-NRB

1

0

51

1.96%

VirD2-Cas9 + mT-NRB

1

0

63

1.58%

Cas9 + mT-NRB

3

1

186

1.61%

Cas9 + mT-RB

2

1

128

1.56%

  1. The successful editing rate was calculated by the exact number of plants showing the proper conversion of the wild-type ALS allele to the herbicide-resistant ALS allele in each set of regenerated plants
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