Fig. 3: RHO expression is found in L75Pfs organoids following viral transduction with an NRL-expression cassette.
From: Investigating cone photoreceptor development using patient-derived NRL null retinal organoids
a–h Confocal images from L75Pfs organoids transduced with a control lentivirus carrying a pgkGFP expression cassette. GFP (c) was found in ARR3+ (b) cones; NRL (g) was not detected, and S-opsin+ cones (f) were localized throughout the ONL and no RHO (k) expression was detected, as expected in the absence of ectopic WT NRL expression. m–x Confocal images from L75Pfs organoids transduced with lentivirus carrying a pgkNRL expression cassette reveal patches of NRL expression within the ONL (o and s) and no NRL co-expression with S-opsin (n and r; merges in p and t). However, ectopically expressed NRL does co-express with RHO (q; merge in t), a rod marker that was never present in control transduced L75Pfs organoids. In pgkNRL-transduced organoids, RHO (w) did not co-localize with the cone marker ARR3 (v; merge in x). Scale bars = 25 μm.
