Fig. 5: Confinement of the PI(3,4,5)P3-enriched region through PI(4,5)P2-induced accumulation of PTEN on the plasma membrane. | Communications Biology

Fig. 5: Confinement of the PI(3,4,5)P3-enriched region through PI(4,5)P2-induced accumulation of PTEN on the plasma membrane.

From: Single-molecule imaging of PI(4,5)P2 and PTEN in vitro reveals a positive feedback mechanism for PTEN membrane binding

Fig. 5

a–c Representative confocal microscopic images of Dictyostelium discoideum pten-null cells expressing PTEN-Halo (a), PTENR47A-Halo (b), and PTENN4-Halo (c) labeled with TMR (magenta) and PHAkt/PKB-EGFP (green). The cells were treated with 5 μM latrunculin A and 4 mM caffeine. Scale bars, 5 μm. d–f Fluorescence intensity distributions of PHAkt/PKB-EGFP on the plasma membrane of pten-null cells expressing PTEN (d, n = 10 cells), PTENR47A (e, n = 10 cells), or PTENN4 (f, n = 19 cells) quantified along the cell periphery in a–c. g A schematic diagram describing the calculation of the anisotropy. h Anisotropy quantified in b. P = 0.00 for all combinations of cell types by Tukey–Kramer test. i Inverse linear correlation between the dissociation rate constant of PTEN and anisotropy of the PI(3,4,5)P3 distribution. Data are mean ± SD.

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