Fig. 7: Fmr1 KO neurons are more sensitive to trifluoperazine treatment.

DIV 14 wild type (WT) (a) and Fmr1 KO neurons (b) were treated with trifluoperazine (TFP) at various concentrations (as indicated). Following the 1 h treatment, levels of pAkt, total Akt, and β-actin were determined by Western blot. The level of pAkt is normalized to the level of total Akt. The relative level of pAkt in the vehicle-treated control group is defined as 1, and all samples are normalized to this group. The p value between the indicated group and the vehicle-treated control group was determined by one-way ANOVA (F5,12 = 17.777, p = 0.000 for a; F5,12 = 33.545, p = 0.000) for (b) followed by post hoc Tukey’s test.