Fig. 2: scRNAseq reveals targets of glucocorticoid signaling.

a Venn-diagram depicting overlap of DEG supersets from scRNAseq and bulk RNAseq. b Heatmap depicting the mean log-scaled expression for scRNAseq-unique DEGs in the scRNAseq. Heatmap is split into three groups based on the pattern of the Dex response; top = downregulated DEGs, middle = progressively upregulated DEGs, bottom = early induced DEGs. c Heatmap depicting Log2FC for scRNAseq-unique DEGs in the bulk RNAseq. Heatmap is in the same order and groups as in b. d Pie charts showing the proportions of each gene group from a that have 1 or 2+ GR peaks within 50 kb of their annotated TSSs. e Scaled Log10 expression (box and scatter plots) and ratio of expressing cells (black dot/line plot) for ZNF703 in scRNAseq. f Independent RT-QPCR validation of ZNF703 expression in response to Dex. Box plot represents ZNF703 expression relative to the geometric mean of GAPDH, ACTB, and TUBA1B. Horizontal edges and lines in boxes represent three biological replicates and “x” represents the mean * = p-value < 0.05 compared to EtOH. g Browser shot of GR ChIP-seq coverage at the ZNF703 locus. h GR ChIP-QPCR validation of GR peaks around the ZNF703 locus. Horizontal edges and lines in boxes represent three biological replicates and “x” represents the mean and ** = p-value < 0.01 compared to corresponding IgG enrichment.