Fig. 5: Heterogeneity and correlation in GR target gene expression patterns. | Communications Biology

Fig. 5: Heterogeneity and correlation in GR target gene expression patterns.

From: Single-cell RNA sequencing reveals a heterogeneous response to Glucocorticoids in breast cancer cells

Fig. 5

a Heatmap depicting pairwise Pearson correlations between scRNAseq DEGs. Vertical bars at the left side of the heatmap depict 6 de novo clusters derived by k-means clustering, as well as the direction in which each DEG was regulated by Dex treatment (UP = upregulated by Dex, DOWN = downregulated by Dex). b Heatmaps depicting the mean scaled-log expression levels in the scRNAseq for each gene cluster in a. c, d tSNE plots designed to mimic co-localization in immunofluorescent images. In the merged image, dark blue indicates a high level of co-expression. R2 value represents the Pearson correlation of the two genes. c PNMT (cyan) and KCNMA1 (magenta) scaled-log expression modeled on a tSNE plots. d DDIT4 (cyan) and NET1 (magenta) scaled-log expression modeled on a tSNE plots.

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