Fig. 2: Co-translocational unfolding is memoryless.
From: Free-energy landscapes of membrane co-translocational protein unfolding
a At a constant voltage of +80 mV (upper left panel), V5-C109-poly(dC)40 was driven into the αHL pore. The intermediate (I) unfolded and was translocated through the pore (T). The lifetimes of I (n = 53) were fitted to an exponential distribution, yielding kI→T = 1.1 s−1 ± 0.1 s−1. b With the same α-HL pore used in a), V5-C109-poly(dC)40 was captured at a higher potential (+120 mV). When I appeared the potential was reduced to +80 mV (asterisk). The lifetimes of I* after the step to +80 mV (n = 98) were fitted to an exponential distribution, yielding kI*→T = 1.05 ± 0.04 s−1.
