Table 1 Comprehensive quantitative lipidomics analysis using liquid chromatography–selected reaction monitoring–mass spectrometry (LC-SRM-MS).

From: The COX-2/PGE2 pathway suppresses apical elimination of RasV12-transformed cells from epithelia

 

MDCK alone

RasV12 alone

Average of alone cultures

Mix culture

P value

Prostaglandin E2

149,032.0

218,274.7

183,653.3

333,123.3

0.00011

Prostaglandin A2

6590.7

8940.3

7765.5

13,786.3

0.00034

17-Hydroxy docosahexaenoic acid

1098.0

2104.7

1601.3

3390.7

0.013

11-HETE

1476.7

5309.0

3392.8

6840.3

0.013

8-Iso prostaglandin E2

5696.7

6452.7

6074.7

11,690.7

0.043

6-Keto prostaglandin F1α

5651.0

11,411.3

8531.2

12,791.0

0.048

Prostaglandin F2α

7913.0

22,378.7

15,145.8

22,196.0

0.087

Oleoyl ethanolamide

1979.3

1353.7

1666.5

4346.7

0.132

Prostaglandin D2

1260.3

6405.3

3832.8

4835.0

0.474

  1. The values for MDCK alone, RasV12 alone or Mix culture indicate the mean of chromatographic peak area for the corresponding SRM transitions from the triplicate samples. P values between ‘Average of alone cultures’ and ‘Mix culture’ were calculated using unpaired t test.
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