Fig. 3: Sensitivity of fluorescent chelator probes for HisQuick-PAGE detection.
From: Ultrafast in-gel detection by fluorescent super-chelator probes with HisQuick-PAGE
a Detection of different histidine tags (Hisn) fused to the maltose-binding protein (MBP) (2 µg, 45 pmol) using trisNTAAlexa647 or hexaNTAAlexa647 (0.45 µM, 7 pmol) by non-reducing SDS-PAGE analysis. b Non-reducing SDS-PAGE detection limit of MBP-His12 by Coomassie or hexaNTAAlexa647 and shift of labeled protein. c HisQuick-PAGE of MBP-His12 (2.5 µg, 60 pmol) incubated at 95 °C (10 min) in the presence of increasing concentrations of reducing agent (DTT, 0–250 mM) prior to hexaNTAAlexa647 (0.45 µM, 7 pmol) labeling.
