Fig. 4: Specific and sensitive detection of recombinant proteins in cell lysates by HisQuick-PAGE.
From: Ultrafast in-gel detection by fluorescent super-chelator probes with HisQuick-PAGE
a Different amounts of His-tagged MBP-His12 10 ng–5 µg (0.23–115 pmol) in E. coli lysate (2.5 µg of total protein) revealed the detection limit of hexaNTAAlexa647 (0.45 µM, 7 pmol). Prolonged exposure visualized 10 ng (230 fmol) of MBP-His12. b Detection of MBP-His12 using the same SDS-PAGE conditions by immunoblot and two different anti-His antibodies.
