Table 1 Detection limits of reference protein MBP-His12.
From: Ultrafast in-gel detection by fluorescent super-chelator probes with HisQuick-PAGE
Detection system | Detection limit (µg) | Detection limit (pmol) |
|---|---|---|
InstantBlueTM | 0.3–0.5 | 7–12 |
Anti-polyHistidine mAb/mouse/Sigma-Aldrich | 0.10–0.15 | 2–3 |
Anti-6X His tag® antibody (HRP)/rabbit/Abcam | ∼0.01 | ∼0.2 |
HisQuick-PAGE | ∼0.01 | ∼0.2 |
