Fig. 3: ReWs are associated with the upregulation of cardiac-specific gene expression. | Communications Biology

Fig. 3: ReWs are associated with the upregulation of cardiac-specific gene expression.

From: Circulating re-entrant waves promote maturation of hiPSC-derived cardiomyocytes in self-organized tissue ring

Fig. 3

a PCA of SOTRs with or without ReWs based on RNA-sequencing data. b Heatmap showing hierarchical clustering of the correlation matrix resulting from comparison of expression values for each sample. Correlations calculated using Spearman’s correlation. c Heatmap showing the relative expression levels (z-score) of 349 genes from SOTRs with ReWs (1 and 2 ReWs) as compared with SOTRs with zero ReW. d GO categories significantly enriched for genes upregulated in SOTRs with two ReWs as compared with SOTRs with zero ReW. Genes with fold changes >1.5 and a P < 0.05 were analyzed. The enriched terms are listed. e Representative confocal images of SOTRs with zero, one, or two ReWs on day 14. Cardiomyocytes were stained with anti-β-MHC (red) and DAPI (blue). Scale bar represents 50 µm. f Quantification of β-MHC levels in SOTRs on day 14. The total integrated fluorescence of β-MHC acquired from confocal imaging was normalized to that of SOTRs with zero ReW (mean ± s.d.; n = 4 biologically independent samples). **P < 0.01 (ANOVA). g The expression of cardiac-specific genes (qPCR) in SOTRs 14 days after cell seeding (mean ± s.d.; n = 4 biologically independent samples). *P < 0.05; **P < 0.01; ***P < 0.001 (ANOVA).

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