Fig. 2: Single-cell characterizations of fracture-hosted microbial populations.
From: Deep microbial proliferation at the basalt interface in 33.5–104 million-year-old oceanic crust
Scanning electron microscopic (SEM) image of a mineral-filled fracture in U1365E-8R4 (a). Confocal laser microscopy image of SYBR Green I-stained microbial cells (b). SEM image of a FIB-derived thin section of U1365-8R4 with a square region (∼10 × ∼10 μm2) analyzed by the JAMSTEC NanoSIMS (c). NanoSIMS images of 12C14N− (d), 31P− (e), 32S− (f), 28Si− (g), and 56Fe16O− (h) with intensity color contours. Overlays are shown from the Ga ion image of the FIB section in black and white and the NanoSIMS images of 12C14N− in blue, 31P− in green, and 32S− in red (i). Dashed rectangles and an arrow show regions presented in the following figures.
