Fig. 2: At Miro2 colocates to mitochondria and affects their morphology and dynamics.
From: Miro2 tethers the ER to mitochondria to promote mitochondrial fusion in tobacco leaf epidermal cells
a–b Spinning disc confocal microscopy images of tobacco leaf epidermal cells transiently expressing the mitochondrial inner membrane marker alone (a, magenta), or with GFP-AtMiro2 (b, green). Hatched box overlay indicates the area displayed in zoom below. Scale bar is 5 μm. c Bar chart showing mean and SEM for mitochondria number per cell region (black bar), area (μm2, grey bar) and circularity (white bar) in cells coexpresing GFP-AtMiro2 WT with mitochondria marker, relative to cells expressing mitochondria marker only. For the mitochondria marker only sample, n = 229 from 24 cells. GFP-AtMiro2-expressing cells, n = 89 from 26 cells. d Bar chart showing mean with SEM for average mitochondrial speeds (μm s−1). The movement of individual mitochondria was tracked in 24–26 cells with a total of 770 marker only organelle tracks and 120 AtMiro2 organelle tracks. Data for c and d were taken from three independent experiments and analysed using a t test with Welche's correction, **p < 0.02. Individual data-points for c and d are plotted in Supplementary fig. 2.
