Fig. 3: Mutation of AtMiro2’s GTPase domain does not affect mitochondrial targeting but does affect mitochondrial morphology and motility.
From: Miro2 tethers the ER to mitochondria to promote mitochondrial fusion in tobacco leaf epidermal cells
a Schematic showing domain organisation of A. thaliana Miro2. The location of the single amino acid mutations introduced into each GTPase domain are indicated, as is an alignment with the corresponding residues in A. thaliana Miro1, S. cerevisiae Gem1p and H. sapiens Miro1 and Miro2 and the corresponding orthologous functional GTPase mutations as previously published27,28,30. b–c Spinning disc confocal images of tobacco leaf epidermal cells transiently coexpressing mitochondria inner membrane marker (magenta) with GFP-AtMiro2 SSNN (green, b), or GFP-Miro2 KKVV (green, c). Scale bar, 5 μm. d Bar chart showing mean and SEM for mitochondria number per cell region (black bar), area (μm2, grey bar) and circularity (white bar) in the mutants relative to AtMiro2 WT mitochondria. e Bar chart showing average mitochondria speeds (μm s−1). Analysis for d and e are derived from 24 and 26 cells from three independent experiments. For the morphological analysis in d, GFP-AtMiro2 n = 89, GFP-AtMiro2 SSNN n = 216, GFP-AtMiro2 KKVV n = 104 organelles. For movement analysis in e, GFP-AtMiro2 n = 120, GFP-AtMiro2 SSNN n = 566, GFP-AtMiro2 KKVV n = 149 organelle tracks. Data were analysed using a t test with Welche’s correction, **p < 0.01. Individual data-points for d and e are plotted in Supplementary fig. 3.
