Fig. 4: Mitochondrial tethering to the ER is affected by AtMiro2.
From: Miro2 tethers the ER to mitochondria to promote mitochondrial fusion in tobacco leaf epidermal cells
Bar chart showing the mean percentage of events: endoplasmic reticulum (ER) was observed to either follow (black), or detach from (white), an optically trapped mitochondrion. In cells expressing either mCherry mitochondria marker, AtMiro2 WT, SSNN, or KKVV with an ER marker, an automated routine (maintaining a set trapping laser power of 40 mW, a set distance of 6 μm and a set speed of 6 μm s−1) was used to trap and move 64 independent mitochondria from 9 to 12 biological replicates from three independent experiments. Cells were treated with latrunculin b. Error bars are SEM. Data were analysed using a Welche’s t-test of weighted means, **p < 0.01. Individual data-points are plotted in Supplementary fig. 4.
