Fig. 3: AFF4 interacts with H3K27ac through its C-terminal region.
From: Acetylation of histone H3K27 signals the transcriptional elongation for estrogen receptor alpha
a Histone H3K27ac peptide pull-down assay. 293 T cell lysate with overexpressed AFF4 (HA-tagged) was used for pull-down. The blot with degraded forms of AFF4 was exposed for an extended time. Histone peptides used for the pull-down assay were stained with GelCodeTM Blue protein stain. b A schematic summary of AFF4 deletion mapping to determine the H3K27ac-interacting region. The regions mediating the interaction between AFF4 and other SEC subunits were indicated by different colors. The key results from the peptide pull-down assay were shown in c–e. In all experiments shown in this figure, 293 T cell lysate with overexpressed AFF4 (HA-tagged) was used for pull-down. f Structure of the AFF4 homodimerization domain. A cartoon representation of the AFF4 C-terminal dimerization domain is depicted, with one monomer shown in magenta and another in cyan. The dashed-lines represent disordered loop segments encompassing residues 1033–1040 and 1047–1080. Phe1103 is shown as a stick model.
