Fig. 5: Analysis of the AFF4/H3K27ac cistrome in MCF7 cells.
From: Acetylation of histone H3K27 signals the transcriptional elongation for estrogen receptor alpha
a Distribution of the distances between AFF4 binding sites to transcription start site (TSS) on the AFF4/H3K27ac co-binding peaks. The Y-axis displays the number of genes associated with each binding site. b Gene ontology of top enriched KEGG pathways on genes associated with these peaks. c Top enriched motifs associated with AFF4/H3K27ac co-binding peaks. d Co-immunoprecipitation (co-IP) experiment to determine the interaction between AFF4 and TFAP2C. 293 T cells were transiently transfected with expression vectors for TFAP2C and AFF4. Reciprocal IP was performed 48 hours later. Input, 3%. e ChIP-qPCR to determine the recruitment of TFAP2C, AFF4, H3K27ac, Cyclin T1, and RNA polymerase II to ESR1 gene. Primers for TSS region of ESR1 gene and primers for an intergenic region on 3′ of ESR1 gene were used for qPCR. The error bars were shown as SD from biological triplicates, n = 3.
