Fig. 4: HSA lacking L585 shows decreased cellular recycling and serum half-life in mice.
From: An intact C-terminal end of albumin is required for its long half-life in humans
a HERA showing relative cellular recycling of WT HSA and L585X. Equal amounts of each variant (1000 nM) were incubated with HMEC1 cells for 4 h, followed by extensive washing and another 4 h incubation step before sample collection. The amounts of recycled WT HSA and L585X were quantified by ELISA, and the obtained data are shown as mean ± s.d. of three independent experiments where n = 3. ****p < 0.0001, by unpaired Student’s t test. b, c Elimination curves of WT HSA (open circle) and L585X (filled circle) in hFcRn transgenic mice. Tg32-Alb−/− mice received 4 mg/kg HSA via intraperitoneal injection (IP) on day 0 (n = 4) (b). Tg32 mice received 2 mg/kg HSA via intravenous injection (IV) on day 0 (n = 5) (c). The serum levels of HSA are presented as percentage remaining in the circulation compared to day 1. The curves represent the mean ± s.d of four or five mice. Mean β-phase half-lives (t1/2) ± s.e.m are shown in days. *p = 0.0245, **p = 0.0023, by unpaired Student’s t test.
