Fig. 5: Plasma-derived L585X shows reduced binding to hFcRn and a short serum half-life in a patient with acute pancreatitis.
From: An intact C-terminal end of albumin is required for its long half-life in humans
a, b Plasma activity of amylase (upward triangle) and lipase (downward triangle) (a) and the percentage of L585X (b) over time in blood of a patient with acute pancreatitis. The percentage of L585X was calculated from the mass spectra on the total HSA (Supplementary Fig. 5). c ELISA showing binding of equal amounts (225 nM) of WT HSA and plasma-derived L585X to hFcRn at pH 5.5. d SPR sensorgrams showing binding of equal amounts (1000 nM) of plasma-derived WT HSA and L585X injected over immobilized (~2000 RU) hFcRn at pH 5.5. Injections were performed at 25 °C, and the flow rate was 50 μl/min.
