Fig. 3: Identification of Notch target genes in muscle progenitors in differentiation conditions. | Communications Biology

Fig. 3: Identification of Notch target genes in muscle progenitors in differentiation conditions.

From: Prostaglandin EP2 receptor downstream of Notch signaling inhibits differentiation of human skeletal muscle progenitors in differentiation conditions

Fig. 3

a Scatter graph of gene expression levels in differentiating Hu5/KD3 cells with (y-axis) or without DAPT treatment (x-axis). RNA was extracted from the differentiating cells after 4 days DAPT treatment and analyzed by RNA-seq. FPKM: fragments per kilobase of exons per million reads mapped. b Numbers of genes differentially (>2-fold) expressed in differentiating Hu5/KD3 cells with or without DAPT treatment. c Experimental design and qPCR analysis of NOTCH2, NOTCH3, PTGER2, and PTGER4 in Hu5/KD3 cells with or without DAPT treatment. RNA was extracted from the cells at day 0, day 4, and day 7. n = 3 samples/group. Data are shown as mean ± S.D. and analyzed by unpaired two-tailed Student’s t-test. The effect size of Pearson’s r correlation (r) is shown.

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