Fig. 7: Blockage of EP2 signaling promoted differentiation of hiPSC-derived muscle progenitors. | Communications Biology

Fig. 7: Blockage of EP2 signaling promoted differentiation of hiPSC-derived muscle progenitors.

From: Prostaglandin EP2 receptor downstream of Notch signaling inhibits differentiation of human skeletal muscle progenitors in differentiation conditions

Fig. 7

a Experimental design. After 6 weeks myogenic induction, muscle progenitors were FACS-sorted and treated with EP2 agonists or an antagonist. b Representative images of myotubes formed by hiPSC-derived muscle progenitors treated with PGE2 (100 ng/ml), butaprost (1 µg/ml), TG6-10-1 (TG, 10 µM), and DAPT (10 µM). Cells fixed with 4% PFA were stained with MF20 (muscle myosin heavy chain, red) and nuclei (DAPI, blue). Scale bar, 200 µm. c, d Fusion index (%) (c) and cell numbers (/mm2) (d). n = 3 samples/group. Three views/sample were analyzed. Data are shown as mean ± S.D. and compared with controls by one-way ANOVA followed by Dunnett’s multiple comparisons test.

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