Fig. 2: G007-LK can reduce WNT/β-catenin signaling in B16-F10 tumors in C57BL/6 N mice. | Communications Biology

Fig. 2: G007-LK can reduce WNT/β-catenin signaling in B16-F10 tumors in C57BL/6 N mice.

From: Tankyrase inhibition sensitizes melanoma to PD-1 immune checkpoint blockade in syngeneic mouse models

Fig. 2

a Representative quantified protein immunoblot ratios (protein vs. loading control) from whole subcutaneous (s.c.) B16-F10 tumors showing altered expression of TNKS1/2, AXIN1, active form of β-catenin (non-phospho, Ser33/37/Thr41) and β-catenin (total). Mean values are indicated by grey lines. For a and b upon 4 days of treatment with G007-LK diet (n = 8, except n = 7 for AXIN1) compared to controls (n = 8). Two-tailed t-tests are indicated by *(P < 0.05) and two-tailed Mann-Whitney rank sum tests are indicated by ‡(P < 0.01). b Real-time RT-qPCR analyses of WNT/β-catenin signaling target genes (Axin2 and Tcf7). One-tailed t-test is indicated by **(P < 0.01) and one-tailed Mann-Whitney rank sum test is indicated by ‡(P < 0.01). Boxplots show median, first and third quartiles and maximum and minimum whiskers for combined data from two independent measurements with three replicates each are shown.

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