Fig. 3: Feather disulfide bond reduction and up-regulation of reducing agent production-associated genes in feather medium-cultured SCUT-3.
From: The feather degradation mechanisms of a new Streptomyces sp. isolate SCUT-3
a Sulfite detection in SCUT-3-cultured CFM supernatant by BaCl2 precipitation, HCl bubble production, and KMnO4 bleaching tests. b Evaluation of SCUT-3 reducing power by detection of sulfhydryl content release from oxidized GSH, showing that only the living cells, but not its intracellular or extracellular components, exhibited reducing power; n = 3/group. c Sulfite improvement of KerK (pink) and DTNB inhibition of SCUT-3 (red) feather degradation, showing the function of sulfite and sulfhydryl groups in feather disulfide bond breakdown; n = 3/group, *P < 0.05, P-values between groups were obtained by unpaired two-tailed Student’s t-test. d qRT-PCR verification of the up-regulation of sulfite production (cdo1/cdo2/mdeA), sulfite exportation (tauE), and mycothiol synthesis (mshA/mshD) genes (n = 3/group). e The operon structure of glutathione transporter genes (red numbers above gene symbols are log2 FC values for each gene). All data were presented as mean ± SD.
