Fig. 5: Iron uptake and oxygen consumption of feather medium-cultured SCUT-3.
From: The feather degradation mechanisms of a new Streptomyces sp. isolate SCUT-3
a, b Up-regulation of the siderophore synthesis (DhbACEBF), iron transporter (EfeUOB), NADH dehydrogenase 1 (NuoA-N), and ATP synthase (atpIBEFHAGDC) operons; numbers above genes are the log2 FC values for each gene (red > 1.0, pink < 1.0). c qRT-PCR verification of siderophore synthesis and exporter (SbnA, SbnA, catE) and iron transporter (yfiY, yfiZ, yusV) gene expression; n = 3/group. d FeSO4 addition (1 μM) improved degradation of white chicken and duck feathers by SCUT-3, but not brown chicken feathers (NS means no significant, P = 0.52); n = 3/group, *P < 0.05. e qRT-PCR verification of oxygen sensor (AcrB/dosT), NADH dehydrogenase 2 (ndh2), and cytochrome c oxidase (ctaD) gene expression; n = 3/group. f Aeration improved SCUT-3 feather degradation in 1% and 10% CFM; n = 3/group, *P < 0.05. P-values between groups were obtained by unpaired two-tailed Student’s t test. All data were presented as mean ± SD.
