Fig. 3: Crotonylation regulates S. roseosporus metabolism.

a KEGG pathway-enrichment analysis for lysine-crotonylated proteins. b Pyruvate production assays with addition of NaCr or NaAc. S. roseosporus wild type was cultured in TSB supplemented with NaCr (pH 7.4) or NaAc (pH 7.4) with the indicated concentrations for 24 h, and the intracellular pyruvate concentration was determined. P value was calculated with Student’s t test (n = 4). c Morphological phenotype of S. roseosporus wild type on R5 with or without 10 mM NaCr (pH 7.4) for 2 or 6 days. d Daptomycin production in wild type supplemented with 10 mM NaCr. The daptomycin yield was measured after culturing 120 h. Experiments were performed in triplicate. P value was calculated with Student’s t test (n = 3).