Table 2 Plasmids used in this study.
Plasmid | Description | References |
|---|---|---|
pKC1139 | Bifunctional oriT RK2 plasmid for gene deletion | |
pKC1139-ΔprcB/A | prcB/A knockout plasmid based on pKC1139 | This work |
pKC1139-ΔcobB | cobB knockout plasmid based on pKC1139 | This work |
pKC1139-Δhdac | hdac knockout plasmid based on pKC1139 | This work |
pKC1139-Δkct1 | kct1 knockout plasmid based on pKC1139 | This work |
pKC1139-Δglk | glk knockout plasmid based on pKC1139 | This work |
pET32a | Expression vector in E. coli with His tag | Novagen |
pET32a-glk | glk in pET32a | This work |
pET32a-glk (K89Q) | glk (K89Q) in pET32a | This work |
pET32a-glk (K91Q) | glk (K91Q) in pET32a | This work |
pET32a-glk (K8991Q) | glk (K8991Q) in pET32a | This work |
pET28a | Expression vector in E. coli with His-tag | Novagen |
pET28a-cobB | cobB in pET28a | This work |
pET28a-kct1 | kct1 in pET28a | This work |
pUT18 | Vector for bacterial two-hybrid system | This work |
pKT25 | Vector for bacterial two-hybrid system | Euromedex |
pUT18-zip | Control plasmid for bacterial two-hybrid system | Euromedex |
pKT25-zip | Control plasmid for bacterial two-hybrid system | Euromedex |
pUT18-glk | glk in pUT18 | Euromedex |
pKT25-cobB | cobB in pKT25 | This work |
pKT25-kct1 | kct1 in pKT25 | This work |
pSN7 | Overexpression integrative shuttle vector containing ermEp* with 3×FLAG tag at N terminus and 18×His tag at C terminus | |
pSN7-glk | glk in pSN7 | This work |
pSN7-glk (K8991Q) | glk (K8991Q) in pSN7 | This work |
pSN7-glk (K8991R) | glk (K8991R) in pSN7 | This work |
