Fig. 3: Influx: FQs have different capacities of membrane translocation measured with SICARIN.100A. | Communications Biology

Fig. 3: Influx: FQs have different capacities of membrane translocation measured with SICARIN.100A.

From: The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux

Fig. 3

SICARIN.100A was obtained from the accumulated concentrations (molecules/cell) in the efflux-deficient mutant AG100A for 5-min incubation with 5 µM of FQs. Data are represented by a box-and-whisker plot (the ends of the box are the upper and lower quartiles, the median is marked by a black line inside the box, and the whiskers are the two lines outside the box that extend to the highest and lowest measurements). Substituents R1, R7, and R8 of the FQ structures are indicated in the table under each corresponding FQ. ANOVA and Tukey’s post hoc tests were performed to determine differences between FQs (n = 32 biologically independent samples, ω²=0.69, degree of freedom = 8). ***P < 0.001; **P < 0.01; *P < 0.05. Data normality was checked by the Shapiro–Wilk test, and homogeneity of variance by the Fligner–Killeen test.

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