Fig. 5: KH-3 inhibits breast cancer cell migration and invasion and promotes CDH1 expression. | Communications Biology

Fig. 5: KH-3 inhibits breast cancer cell migration and invasion and promotes CDH1 expression.

From: Targeting the interaction between RNA-binding protein HuR and FOXQ1 suppresses breast cancer invasion and metastasis

Fig. 5

a–c Scratch assay in MDA-MB-231 and SUM159 cells treated with DMSO or KH-3. a, b Representative images of cell migration at 0 and 24 h after scratching with indicated treatment in MDA-MB-231 (a) and SUM159 (b) cells, scale bars: 50 μm. c Wound widths in two cell lines 24 h after scratching and treatment (***P < 0.001, t-test, n = 3). d–f Invasion assay in MDA-MB-231 and SUM159 cells treated by DMSO, KH-3B or KH-3. d, e Representative images of stained invaded cells with indicated treatment in MDA-MB-231 (d) and SUM159 (e) cells, scale bars: 200 μm. f Invaded cell numbers per image in both cell lines with indicated treatment (***P < 0.001, one-way ANOVA, n = 6). g The heatmap view of PCR pathway array focusing on invasion and metastasis related genes. The relative mRNA levels were presented as z score, each treatment was triplicated. h CDH1 luciferase reporter assay in HEK 293FT cells treated by DMSO, KH-3B or KH-3. Values are mean ± SD from n = 4 independent experiments (**P < 0.01, ***P < 0.001, two-way ANOVA).

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