Fig. 5: Cellular fractionation following expression from standard pET28a plasmid versus TIR-2 + T7pCONS plasmid.
From: Improved designs for pET expression plasmids increase protein production yield in Escherichia coli
Western blot quantification of fractionations of BL21(DE3) pLysS (total, soluble and insoluble) following expression of a sfGFP, b MTH1 or c Neil3 from either the standard pET28a plasmid (pink bars) or pET28a-TIR-2+T7pCONS plasmid (green bars). In all examples, application of the combined TIR-2+T7pCONS resulted in enhanced levels of target proteins. Furthermore, use of TIR-2+T7pCONS did not alter the solubility profile for each target. Representative Western blots are shown on the right panels with each protein marked. Data are presented as mean ± s.d. (n = 3).
