Fig. 6: Subcapsular engraftment of embryonic kidney tissue results in stromal over-abundance.
From: Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids
a NSG mice were engrafted with embryonic kidney tissue using two distinct strategies: E15.5 whole embryonic kidneys were subcapsularly engrafted and harvested after 1 or 2 months, or nephrogenic zone cells were isolated from E17.5 kidneys, aggregated to organoids, subcapsularly engrafted and harvested after 2 weeks. b–e Tissue from E15.5 kidney graft. b H&E staining reveals extensive graft growth. c WT1 staining of podocytes shows abundant glomeruli and LTL staining for proximal tubules that these are sparse in the graft tissue. d CD31 staining of endothelial cells shows that glomeruli are vascularized. e Podocalyxin (Podxl) staining outlines glomeruli and PDGFRβ staining reveals abundant stromal cells between glomeruli. f–h Tissue from NZC organoid graft. f Example of NZC organoid at the time of engraftment. g WT1 staining for podocytes reveals abundant glomeruli. h CD31 staining for endothelial cells shows vascularization of the graft tissue. i PDGFRβ staining for stromal cells shows that these are abundant in the graft tissue.
