Table 1 List of antibodies/lectins.
From: Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids
Name | Catalog/clone number | Source | Dilution |
|---|---|---|---|
Cdh1 | ab11512 | Abcam | 1:100 |
Podxl | AF1658 | R&D systems | 1:100 |
LTL | B-1325 | Vector | 1:200 |
Brn1 | sc-6028-R | Santa cruz | 1:100 |
GATA3 | 5852S | Cell signaling Tech. | 1:100 |
DBA | B-1035 | Vector | 1:200 |
WT1 | ab89901 | Abcam | 1:50 |
Anti-human CD31 | 3528 | Cell signaling Tech. | 1:50 |
Anti-mouse CD31 | 550274 | BD Pharmingen | 1:50 |
Pdgfrβ | ab32570 | Abcam | 1:100 |
Meis1 | 39795 | Active Motif | 1:100 |
Hnf4a | C11F12 | Cell signaling | 1:100 |
Lhx1 | 4F2-c | Developmental Studies Hybridoma Bank | 1:200 |
Emcn | ab45771 | Abcam | 1:100 |
SMA | C6198 | Sigma | 1:200 |
THP | J65429 | Alfa Aesar | 1:200 |
KRT8 | TROMA-1-c | Developmental Studies Hybridoma Bank | 1:200 |
Renin | ab212197 | Abcam | 1:100 |
HuNu | MAB1281B | Millipore | 1:50 |
Collagen I | 600-401-103-0.1 | Rockland | 1:200 |
Collagen IV | 600-401-106-0.1 | Rockland | 1:200 |
Laminin | L9393 | Sigma | 1:200 |
Six2 | 11562-1-AP | Proteintech | 1:100 |
Donkey anti-Rabbit IgG, Alexa Fluor 488 | A-21206 | Thermo Fisher Scientific | 1:200 |
Donkey anti-mouse IgG, Alexa Fluor 488 | A32766 | Thermo Fisher Scientific | 1:200 |
Donkey anti-rat IgG, Alexa Fluor 488 | A-21208 | Thermo Fisher Scientific | 1:200 |
Streptavidin, Alexa Fluor 488 | S11223 | Thermo Fisher Scientific | 1:200 |
Donkey anti-Rabbit IgG, Alexa Fluor 568 | A10042 | Thermo Fisher Scientific | 1:200 |
Donkey anti-Goat IgG, Alexa Fluor 568 | A-11057 | Thermo Fisher Scientific | 1:200 |
Goat anti-Mouse IgG1, Alexa Fluor 568 | A-21124 | Thermo Fisher Scientific | 1:200 |
Streptavidin, Alexa Fluor 568 | S11226 | Thermo Fisher Scientific | 1:200 |
Donkey anti-Rabbit IgG, Alexa Fluor 647 | A-31573 | Thermo Fisher Scientific | 1:200 |
Streptavidin, Alexa Fluor® 647 | S21374 | Thermo Fisher Scientific | 1:200 |
