Fig. 1: Effect of local disruption of otolith organs on hypergravity (2g)-induced hypothermia.

a, b Averaged data of the body temperature (BT, magenta) and activity (blue) prior to and during the 2g load (n = 8) in mice with a vestibular lesion (VL) and sham-operated mice (Sham). The horizontal open and closed squares indicate the light and dark periods, respectively. c, d Summarized data of the changes in BT (c) and activity (d) induced by 2g load in the Sham (n = 8) and VL (n = 8) mice. Two-way ANOVA with either Bonferroni’s or Tukey’s post-hoc test. * or † vs. averaged value of pre-loading in the Sham or VL mice, respectively; ‡ Sham vs. VL. e Summarized data of the changes in BT during either on-axis or off-axis rotation (n = 7 for each). Two-way ANOVA with Bonferroni’s multiple comparisons test. f Hypergravity (2g) was created within 10 min, 6 h, 24 h, and 48 h. The BT and activity were recorded for 48 h (from 11 am to 11 am). The graph with a line indicates the BT, while the bar graph depicts activity. The horizontal bar under the numbers (Time) represents the light-and-dark cycle. g Summarized data of BT induced by a slow increase in gravity from 1g to 2g in mice (n = 4). Two-way ANOVA with the Tukey’s and Bonferroni’s multiple comparisons tests. For all statistical analyses, single, double, or triple significant symbols indicate P < 0.05, P < 0.01, or P < 0.001, respectively. Detailed information on the statistical analyses is reported in Supplementary Table 2.