Fig. 3: Manipulation of VGLUT2- and VGAT-expressing neurons located in the vestibular nuclear complex (VNC) using both the optogenetics tool. | Communications Biology

Fig. 3: Manipulation of VGLUT2- and VGAT-expressing neurons located in the vestibular nuclear complex (VNC) using both the optogenetics tool.

From: VGLUT2-expressing neurons in the vestibular nuclear complex mediate gravitational stress-induced hypothermia in mice

Fig. 3

a Representative images of either ChR2 or eArch expression in VGLUT2- and VGAT-expressing neurons in the VNC. The scale bar is 100 μm. b Photostimulation of either the ChR2 (blue) or the eArch (green) in VGLUT2- and VGAT-expressing neurons located in VNC-induced body tilt. The photostimulation was applied in the unilateral VNC. Please see the Supplementary Movie 1. c, d Summarized data of the changes in body tilt angle induced by photostimulation in either VGLUT2-Cre (c) or VGAT-Cre (d) mice. One-way ANOVA with Tukey’s post-hoc test. e, f Summarized data of the body tilt angle induced by changes in the frequency of photostimulation in either VGLUT2-Cre (e) or VGAT-Cre (f) mice. Two-way ANOVA with Tukey’s multiple comparisons test. * vs. 10 Hz and 20 Hz, † vs. Control. For all statistical analyses, single, double, or triple significant symbols indicate P < 0.05, P < 0.01, or P < 0.001, respectively. Detailed information on the statistical analyses is reported in Supplementary Table 2.

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