Fig. 7: Exposure to the 2g environment attenuated the subsequent hypothermic response. | Communications Biology

Fig. 7: Exposure to the 2g environment attenuated the subsequent hypothermic response.

From: VGLUT2-expressing neurons in the vestibular nuclear complex mediate gravitational stress-induced hypothermia in mice

Fig. 7

a Time-dependent changes in the body temperature (BT) following the injection of clozapine N-oxide (CNO) in VGLUT2-Cre mice (n = 10). The viral vector, AAV–CAG–FLEX–hm3D(Gq)–mCherry (AAV–hm3D(Gq)–mCherry), was injected in the VNC bilaterally. Each value was obtained by averaging the values every 30 min. One-way ANOVA with the Tukey’s multiple comparisons test; *P < 0.05 vs. pre. b A schema of the assessment of the effect of CNO administration on the following 2g-induced hypothermia in VGLUT2-Cre and VGAT-Cre mice. c, d Summarized data of the changes in BT (c) and activity (d) induced by 2g load in VGLUT2-Cre and VGAT-Cre mice with either advanced CNO injection or delivery of its vehicle (n = 8 in each group). Two-way ANOVA with Tukey’s post-hoc test. * vs. averaged value of pre-loading in VGLUT2-vehicle, VGAT-vehicle, and VGAT-CNO; † VGLUT2-vehicle vs. VGLUT2-CNO. e Exposure to 2g was applied for 9 h in C57BL/6 J mice (n = 8). The hypothermic response for 3 h was evaluated two days after the 2g experience. The averaged time-dependent changes in the BT (magenta line) and activity (blue line) are shown. f The effect of the 2g load on the following 2g-induced hypothermia in C57BL/6 mice. A paired t-test was applied for the statistical analysis. For all the statistical analyses, single, double, or triple significant symbols indicate P < 0.05, P < 0.01, or P < 0.001, respectively. Detailed information on the statistical analyses is reported in Supplementary Table 2.

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