Fig. 4: TRPM4 channels in accelerating GrCs. | Communications Biology

Fig. 4: TRPM4 channels in accelerating GrCs.

From: Parameter tuning differentiates granule cell subtypes enriching transmission properties at the cerebellum input stage

Fig. 4

a The voltage traces show the GrC response to a 10 pA step current in an accelerating and strong-adapting GrCs. A five-steps depolarizing protocol is applied to uncover DISC. In the accelerating GrC, DISC typically appears as a delayed burst of rapid events. DISC cannot be longer elicited after perfusing a specific TRPM4 channel blocker, 9-Phenantrhol (100 µM). No DISC is visible in the strong-adapting GrC (all currents low pass filtered at 500 Hz). b The average area of DISC transients shows a significant increase after the depolarizing protocol in accelerating GrCs (**p = 0.02, n = 15, paired t-test,) but not in strong-adapting granule cells. c Raw average current traces for accelerating and strong-adapting GrCs are shown in light gray. In accelerating GrCs (n = 15) the DISC currents are still evident at 4 s, while no net current changes are visible in strong-adapting GrCs even 6 s after the impulse train (n = 27). The corresponding filtered average traces (orange and blue) are shown superimposed (adjacent-averaging smoothing).

Back to article page