Fig. 5: TRPM4 expression in GrCs. | Communications Biology

Fig. 5: TRPM4 expression in GrCs.

From: Parameter tuning differentiates granule cell subtypes enriching transmission properties at the cerebellum input stage

Fig. 5

a Fluorescence image of a slice cut from the cerebellar vermis (220 μm thick) treated with polyclonal antibodies directed against TRPM4 (magenta) and PAX6 (green) and stained with Hoechst to visualize cell nuclei (blue). TRPM4 expression is visible as a thin-outline around GrCs membrane, while PAX6 and Hoechst fluorescence are confined to the nuclear region (dotted arrow). Note the typical rounded shape of the GrCs and the nucleus occuping most of the cytoplasm. Alternatively, either PAX or Hoechst signals dominate nuclear fluorescence (arrows show two examplar cells) (x40 objective magnification, scale bar 5 μm). b A GrC was selected from a (white circle) and shown at x2 magnification (scale bar 10 μm). The upper panels show the acquisition channels separately (TRPM4, PAX6, and Hoechst, respectively), while in the bottom panel the three channels are merged to highlight the coexpression of TRPM4, PAX6, and Hoechst. It should be noted that TRPM4 staining surrounds the nucleus and that some dots can also be observed inside the cell.

Back to article page