Fig. 4: Uptake of HS201 and VP by murine spontaneous BC. | Communications Biology

Fig. 4: Uptake of HS201 and VP by murine spontaneous BC.

From: Heat shock protein 90-targeted photodynamic therapy enables treatment of subcutaneous and visceral tumors

Fig. 4

a nIR signal from breast tumor-bearing MMTV-neu mice injected with HS201 or VP. MMTV-neu mice that developed spontaneous BCs with the size of 5–15 mm in diameter were selected, and administered 25 nmol of HS201 or VP via tail vein. nIR signals from the tumor area were detected using a Pearl Imager over time. The images of two representative mice from each group are shown. b Temporal dynamics of nIR signal intensity from spontaneous tumors by in vivo imaging. The average fluorescence intensities (5 mice for HS201 group and 6 mice for VP group) are shown. Half-life of HS201 and VP signal detected in the tumor site was 42 and 15 h, respectively. Data are expressed as means ± SEM. c Ex vivo imaging of spontaneous tumors in MMTV-neu mice. Mice (5 mice 7 tumors for HS201 group, 4 mice 7 tumors for VP group, and 2 mice 3 tumors for control group) were sacrificed at the 6-h time point after HS201 or VP (25 nmol/mouse) injection. nIR signals from excised tumors are shown in the graph. Kruskal–Wallis test was performed followed by nonparametric Dunnett multiple comparisons. d Flow cytometry analysis of HS201 uptake by breast tumor cells and normal mammary epithelial cells in tumor-bearing MMTV-neu mice. HS201 (100 nmol/mouse) was injected into MMTV-neu mice (three tumor-bearing mice with four tumors and two non tumor-bearing mice) via tail vein. Control mice (one tumor-bearing mouse and one non tumor-bearing mouse) received no compound injection. Mice were sacrificed 6 h after compound injection. Tumors and mammary gland tissues were digested into single cells, acquired by an LSRII flow cytometer and nIR signals from tumor cells and mammary epithelial cells (CD24 positive and CD45 negative cells) were analyzed. Dot plots of tumor and epithelial cells from a representative mouse are shown with percentages of HS201 positive cells. Percentages of HS201-positive cells in four samples of breast tumor cells and four samples of normal mammary epithelial cells are shown as a box plot, and t test was performed.

Back to article page