Fig. 6: Enhanced HS201 accumulation and Hsp90 expression in human BC xenografts after HS201-PDT in vivo. | Communications Biology

Fig. 6: Enhanced HS201 accumulation and Hsp90 expression in human BC xenografts after HS201-PDT in vivo.

From: Heat shock protein 90-targeted photodynamic therapy enables treatment of subcutaneous and visceral tumors

Fig. 6

a Enhanced HS201 accumulation in MDA-MB-231 tumors in vivo after laser exposure. MDA-MB-231 tumor-bearing mice were administered HS201 or VP (25 nmol). The laser irradiation (120 J/cm2/4 min) was applied twice (drug-light interval of 6 and 24 h) at 690 nm wavelength to the mice in HS201-PDT and VP-PDT groups. Whole-body images were acquired by a Pearl Trilogy imaging system at the 700 nm channel overtime and images of representative mice for each group are shown. b Temporal dynamics of nIR signal and tumor:background ratio from MDA-MB-231 tumors by in vivo imaging. The nIR signals detected at the tumor site and shaved and depilated skin around the right thigh were measured. The tumor:background ratio was calculated and plotted in the graph (right panel). Averages ± SEM of nIR signal intensities acquired from five mice per group are plotted in the graph. Red arrows indicate the timing of laser irradiation. c Hsp90 expression in MDA-MB-231 tumors treated with or without HS201-PDT in vivo. MDA-MB-231 tumor-bearing mice were administered HS201 (25 nmol/mouse), irradiated with laser (690 nm, 120 J/cm2/4 min) 6 h after PS injection, and sacrificed at 12-h time point to collect the tumor lysates. Hsp90 and GAPDH expression by tumors of each group was quantified by Western blot. The table shows Hsp90/GAPDH ratio of each group. d Confocal microscope images of excised MDA-MB-231 tumors treated with or without HS201-PDT. MDA-MB-231 tumor-bearing mice were administered with HS201 or VP, followed with or without laser irradiation (690 nm, 120 J/cm2/4 min) 6 h after injection, and sacrificed at 12-h time point. Tumors were fixed overnight in formalin before sectioning. Tissue sections were stained with WGA Alexa Fluor 488 conjugate membrane staining dye and DAPI and observed using a ZEISS LSM880 confocal microscope. nIR signals from photosensitizers (magenta color) are shown at the bottom. Mean fluorescence intensities for tile scanned large tumor sections are shown in each microscope image. Original magnification: Objective 40×. The scale bar indicates 15 μm. The images of full-length blots are available in Supplementary Fig. 13.

Back to article page