Fig. 3: Enzymology of tetracycline inactivation by Tet(X7).

Michaelis–Menten kinetics of tetracycline-inactivating enzyme-mediated degradation of last-generation tetracyclines. a Michaelis–Menten plot of Tet(X) degradation of tigecycline and eravacycline. b Michaelis–Menten plot of Tet(X7) degradation of last-generation tetracyclines. c Apparent Km, kcat, and catalytic efficiencies for the tetracycline-inactivating enzyme-mediated degradation of tigecycline, eravacycline, and omadacycline. d Last generation tetracycline antibiotics tigecycline, eravacycline, and omadacycline. Error bars represent standard deviation for three independent trials.