Fig. 5: The protein stability of SRPK1 was affected by acetylation. | Communications Biology

Fig. 5: The protein stability of SRPK1 was affected by acetylation.

From: SRPK1 acetylation modulates alternative splicing to regulate cisplatin resistance in breast cancer cells

Fig. 5

293T cells were transfected with Myc-tagged SRPK1 (Myc-K1) and Tip60 or HAT-deficient mutant (mut) (a), or transfected with Tip60 and Myc-tagged SRPK1 or Mut7 (b). The cells were then treated with 100 µM CHX for indicated time. The level of Myc-tagged SRPK1 protein was checked by immunoblotting. c 293T cells were transfected with Myc-tagged SRPK1 (K1) or Mut7 and Tip60 as indicated. The cells were then immunostained with the c-Myc antibody. The nuclei were counterstained with Hoechst 33342. Scale bar: 5 µm. d The indicated cells were treated with cisplatin for 24 hours, followed by CHX for the indicated time. The protein level of endogenous SRPK1 was evaluated by immunoblotting. Western blots in (a, b, d) and immunofluorescence in (c) are representative of three experiments with similar results.

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