Fig. 6: Alternative splicing of BCL-2 family genes could be affected by SRPK1 acetylation.

a 231 and 231R cells were treated with indicated concentrations of cisplatin. The alternative splicing of BCL2L1 and MCL-1 was examined by RT-PCR. b In cisplatin-treated 231R cells, the acetylation of SRPK1 was manipulated by the indicated single transfection and co-transfection. The levels of alternatively spliced variants of BCL2L1 and MCL-1 were checked by RT-PCR. The decimals below the gel strips in (a, b) denote the relative abundance of short (S) versus long (L) variants. c 231R cells were co-transfected with the mCherry-fused MCL-1 splicing-sensitive reporter (MCL1-PTC mCherry), Tip60 and SRPK1 or Mut7 as indicated. The mCherry signals were recorded by the fluorescence microscopy and superimposed onto the phase-contrast images. Scale bar: 20 µm. Bars: mean ± SD; n = 5; **p < 0.01 by Student’s t-test. The RT-PCR gel images in (a, b) are representative of three experiments with similar results.