Fig. 2: Spatial expression patterns of HvHKT1;5 haplotypes.
From: Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5
a Sections used for quantification of transcript abundance (after Burton et al., 200447). b Quantification of HvHKT1;5 in root (Tip, 2, 3, and MZ) and shoot (A, B, C, and D) tissues from 12-day-old barley plants. Dark blue—Na+HAP1 (cv. Golden Promise), pale blue = Na+HAP2 (cv. Vivii), red = Na+HAP3 (cv. Morex) differences from after carrying out an ANOVA followed by Tukey HSD within tissue type, same letters in lower case indicate no significant difference using P < 0.05. For boxplots the horizontal bar of the boxplot shows the median, the box delineates the first and third quartile, and the whiskers show ±1.5 × IQR. c In situ localisation of HvHKT1;5 in 2-week-old barley root tissue from cv. Golden Promise, Na+HAP1 (grown without added NaCl). Top: negative controls with no RT (reverse transcription), Middle HvHKT1;5 with c cortex, en endodermis, x xylem, xp xylem parenchyma labelled in red. Bottom Hv18S rRNA (positive control). Scale bars, 100 μm.
