Fig. 1: Severe NE and ER membrane defects observed in lem2Δlnp1Δ cells.
From: Lem2 and Lnp1 maintain the membrane boundary between the nuclear envelope and endoplasmic reticulum
a NE structure of lem2Δlnp1Δ cells. The NE was labeled with Ish1-mCherry. A single-section image (upper left) and a maximum-intensity projected image (upper right) of lem2Δlnp1Δ cells are shown; single-section images are shown for wild-type (WT), lem2Δ, and lnp1Δ cells in the lower panels. N represents the nucleus. Arrows and arrowheads indicate disordered and highly aggregated membrane structures, respectively. Scale bar represents 5 μm. b Quantification of NE defects. Percentages of cells with abnormal NE were calculated from three independent experiments and plotted as mean ± standard deviation. n indicates the number of experiments. Open circles represent the percentage of individual experiment. p Values are from Tukey’s test. **p < 0.01. c Effects on NPC structure. Cut11-GFP was co-expressed with Ish1-mCherry in the WT, lem2Δ, lnp1Δ, and lem2Δlnp1Δ cells, respectively. Arrows indicate an abnormal nuclear membrane. Scale bar represents 5 μm. d Effects on ER structure. GFP-tagged ER retention signal (GFP-ADEL) was co-expressed with Ish1-mCherry in the WT, lem2Δ, lnp1Δ, and lem2Δlnp1Δ cells, respectively. Arrows indicate an abnormal nuclear membrane. Scale bar represents 5 μm. e Effects on Rtn1 localization. Rtn1-GFP was co-expressed with Ish1-mCherry in the WT, lem2Δ, lnp1Δ, and lem2Δlnp1Δ cells, respectively. Arrow shows an abnormal nuclear membrane. Scale bar represents 5 μm.
