Fig. 3: TA and DOP partially abrogate the effect of EPI in inhibiting HaCaT cells migration by affecting cAMP level and F-actin formation.
From: Trace amines produced by skin bacteria accelerate wound healing in mice
a HaCaT cells migration was examined using a similar method as the wound healing assay with the addition of Mitomycin C (10 µg/ml). TA, DOP and ICI (25 µg/ml) enhanced the HaCaT cells migration in the presence of EPI (25 µg/ml). TA and DOP alone did not show any significant effect while ICI boosted the HaCaT cells migration. b HaCaT cells was incubated with EPI (25 µg/ml) and TA, DOP and ICI as positive control (25 µg/ml) separately for 2 h. TA, DOP and ICI were added 30 min prior to the addition of EPI. EPI increased the intracellular cAMP level of HaCaT cells but the addition of TA, DOP and ICI, decreased the cAMP level compared to HaCaT cells treated with EPI alone. c EPI (25 µg/ml) decreased the F-actin level compared to the control. The addition of TA, DOP and ICI (25 µg/ml) in the presence of EPI, increased the F-actin level compared to the treatment with EPI only. For all graphs, each data point is the mean value ± SEM from 3 independent replicates for (a) and from 6 independent replicates for (b), *p < 0.05; **p < 0.01, data were analyzed using Student's t test. Source data are provided as a Supplementary Data 1 file.
