Fig. 1: Jlp global deficiency aggravated UUO-induced kidney fibrosis.
a Representative images (five visual fields for each tissue analyzed) of HE and MTS of renal tissue section from indicated groups (left panel) and quantification of tubular lesion and interstitial fibrosis (right panel). Scale bar, 50 μm (insets, 10 μm). n = 5. b Representative images (five visual fields for each tissue analyzed) of IF staining for α-SMA and FN (green), and IHC staining for collagen-I in the indicated renal tissue sections. Cell nuclei are visualized by co-staining with DAPI. Scale bar, 50 μm.The positive areas of indicated protein were further presented in quantification (Right panel). n = 5. c α-sma, Fn, and Collagen-I mRNA level in the indicated kidney samples were measured by qPCR and normalized by Gapdh mRNA level. Expression of relative amounts of genes was calculated by the comparative CT method (2-△△CT) with the Jlp+/+ sham group normalized to a fold value of 1. n = 5. d Western blotting analyses the expression of indicated proteins in the indicated kidney samples. GAPDH was set as loading control. The indicated band intensity of western blotting was normalized to the relevant band intensity of GAPDH. n = 5. e Representative images (five visual fields for each tissue analyzed) of IF staining for F4/80 (green), and IHC staining for Caspase-3 in the indicated kidney samples (left panel) and quantification of F4/80 and Caspase-3 expression based on IF or IHC staining. Scale bars, 50 μm. n = 5. Quantitative data are expressed as the mean ± s.e.m. Two-way ANOVA was applied for two-group comparisons. NS = no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001.
