Fig. 3: Expression of scaffold protein JLP was decreased in fibrotic kidneys from the UUO model or CKD patients. | Communications Biology

Fig. 3: Expression of scaffold protein JLP was decreased in fibrotic kidneys from the UUO model or CKD patients.

From: A negative feedback loop between JNK-associated leucine zipper protein and TGF-β1 regulates kidney fibrosis

Fig. 3

a Representative images (five visual fields for each tissue analyzed) of IF staining of JLP (green) in the renal cortex from indicated groups, Jlp−/− mice sets as negative control. Scale bars, 50 μm. n = 5 b Representative images (five visual fields for each tissue analyzed) of IHC staining of JLP in kidney from indicated group. Jlp−/− mice sets as negative control. Scale bars, 50 μm. n = 5 c Representative images (five visual fields for each tissue analyzed) of IF staining of JLP (green) in the renal cortex of kidney from healthy human biopsies and individuals with CKD. Ctrl IgG sets as negative control. Scale bars, 10 μm. d Representative images (five visual fields for each tissue analyzed) of IHC staining of JLP in the renal cortex of kidney from healthy human biopsies and individuals with CKD. Ctrl IgG sets as negative control. Scale bars, 10 μm. e Expression of JLP protein by western blotting of tissue lysates from kidney in indicated experimental groups and quantified, n = 5. f Relative expression of Jlp gene from kidney in the indicated groups. Data are normalized to Gapdh mRNA level. n = 5. g Relative Jlp mRNA level was determined by qPCR in normal control kidney samples and kidney samples from individuals with CKD. n = 4. h Expression of JLP protein by western blotting of HK-2 cells lysates in indicated experimental groups (Left panel), and the relative abundance of JLP expression was quantified (Right panel). GAPDH sets as loading control. n = 5. i Expression of JLP protein by western blotting of HK-2 cells lysates in indicated experimental groups (Left panel), and the relative abundance of JLP expression was quantified (Right panel). GAPDH sets as loading control. j Relative Jlp mRNA level was determined by qPCR in HK-2 cells from different groups as indicated. Data are normalized to Gapdh mRNA level. n = 5. Quantitative data are expressed as the mean ± s.e.m. One-way ANOVA test with Bonferroni correction was used. NS = no significant difference, *P < 0.05.

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