Fig. 2: Aged mice were highly susceptible to stress-induced depression.
From: Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice
a Experimental design for treatment with daily 2-h restraint for 5 days (RST5d) or 14 days (RST14d) in young (2M) and aged (18M) mice, and following behavioral tests. b, c Representative tracking plots, time spent in the target and non-target fields (n = 8 mice/group; target field, One-way ANOVA, F(4,35)=7.416, p = 0.0002; non-target field, One-way ANOVA, F(4,35)=7.428, p = 0.0002). d Sociability index (n = 8 mice/group; One-way ANOVA, F(4,35)=7423, p = 0.0002). e Total locomotion in the sociability test (n = 8 mice/group; One-way ANOVA, F(4,35)=2.116, p = 0.0996). f Immobility time in the FST (n = 8 mice/group; One-way ANOVA, F(4,35)=12.97, p < 0.0001). g, h K-Means clustering of all individuals in the sociability test x FST matrix plotting with z-scores (standard deviations) and proportion of each group in the two clusters (cluster1: 87.5% for 2M CON, 87.5% for 2M RST5d, 12.5% for 2M RST14d, 87.5% for 18M CON, and 0% for 18M RST5d; cluster2: 12.5% for 2M CON, 12.5% for 2M RST5d, 87.5% for 2M RST14d, 12.5% for 18M CON, and 100% for 18M RST5d). i, j Expression levels of p47phox and gp91phox transcripts in the hippocampus of indicated groups (n = 6, each; p47phox, One-way ANOVA, F(4,25)=43.24, p < 0.0001; gp91phox, One-way ANOVA, F(4,25)=25.3, p < 0.0001). *p < 0.05 and **p < 0.01. One-way ANOVA followed by a Newman–Keuls post hoc test.
