Fig. 3: Aging- and stress-induced increase of p47phox expression was regulated by AMPK.
From: Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice
a, b Expression levels of Prkaa1, p38α, Akt1, and Pik3ca transcripts in the hippocampus of mice treated with RST14d and their control, and of normal young (2M) and aged (18M) mice (n = 6, each). c Western blot data showing p-AMPK and AMPK levels in the hippocampus of young and aged mice (n = 7, each). d Photomicrographs showing co-localization of p-AMPK and p47phox in CA3 pyramidal neurons of mice. Scale bars, 100 µm (left), 20 µm (right). e Inverse relationship in the expression levels between p-AMPK and p47phox (arrows and arrow heads, respectively) (n = 49 cells). f Western blot data showing p-AMPK levels in HT22 cells treated with GC (400 ng/ml) for 24 h (n = 5, each). g, h p47phox transcript levels and DHE-reactive ROS levels in HT22 cells treated with GC (400 ng/ml) or GC plus AICAR (p47phox, n = 6, each; One-way ANOVA, F(4,25)=16.47, p < 0.0001; DHE, n = 6, each; One-way ANOVA, F(2,15)=10.71, p = 0.0013). i, j p47phox transcript level and DHE-reactive ROS levels in HT22 cells treated with Compound C (1 µM) (p47phox, n = 8, each; DHE, n = 6, each). k A summary of the signaling pathways of p-AMPK and p47phox. *p < 0.05 and **p < 0.01. One-way ANOVA followed by a Newman–Keuls post hoc test.
