Fig. 5: SUV39H1 mediated aging- and stress-induced upregulation of p47phox and gp91phox expression.
From: Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice
a Transcript levels of histone acetyltransferases (HATs: Cbp and p300), histone deacetylases (HDACs: Hdac1, Hdac2, Hdac3, Sirt1, and Sirt7), H3K9 lysine-specific demethylases (H3K9 KDMs: Jmjd2a, Jmjd2b, and Jmjd2c) and H3K9 lysine-specific methyltransferases (H3K9 KMTs: Setdb1 and SUV39H1) in the hippocampus of mice at 2 and 18 months of age (n = 6, each). b–d SUV39H1, Hdac2, and Sirt1 transcript levels in the hippocampus of mice at 2, 7, 14, and 18 months (n = 6, each; SUV39H1, One-way ANOVA, F(3,20)=7.065, p = 0.002; Hdac2, One-way ANOVA, F(3,20)=5.783, p = 0.0051; Sirt1, One-way ANOVA, F(3,20)=2.666, p = 0.0756). e–g Diagram (e) of the hippocampus with the areas (red box) used for the high magnification images (f). Scale bars, 50 µm. Quantification levels (g) (n = 8 mice/group). h–j SUV39H1 transcript levels in the hippocampus of mice treated with RST14d plus siCON, or RST14d plus siPpp2ca as depicted in Fig. 4g; RST14d plus Veh, or RST14d plus AICAR as depicted in Supplementary Fig. 5a; and CC as depicted in in Supplementary Fig. 5g (n = 6, each; h One-way ANOVA, F(2,15)=6.365, p = 0.01; i One-way ANOVA, F(2,15)=12.84, p = 0.0006). k SUV39H1 transcript levels in young mice treated with RST5d or RST14d, and aged mice treated with RST5d, and their control (n = 6, each; One-way ANOVA, F(4,25)=21.4, p < 0.0001). l, m Western blot data showing p-CREB and CREB levels in the HT22 cells treated with AICAR or CC, respectively (AICAR, n = 4, each) (CC, n = 5, each). n Creb, SUV39H1, and p47phox transcript levels in the HT22 cells treated with siCON or siCreb (n = 6, each). o A summary of the signaling pathway of PP2A, p-AMPK, p-CREB, SUV39H1, p47phox, and gp91phox. *p < 0.05 and **p < 0.01. One-way ANOVA followed by a Newman–Keuls post hoc test.
